We believe that most of the matter in the universe is dark, i.e. cannot be detected from the light which it emits (or fails to emit). This is "stuff" which cannot be seen directly -- so what makes us think that it exists at all? Its presence is inferred indirectly from the motions of astronomical objects, specifically stellar, galactic, and galaxy cluster/supercluster observations. It is also required in order to enable gravity to amplify the small fluctuations in the Cosmic Microwave Background enough to form the large-scale structures that we see in the universe today.
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